Microscopic Investigation of Reversible Nanoscale Surface Size Dependent Protein Conjugation

نویسندگان

  • Kazushige Yokoyama
  • Hyunah Cho
  • Sean P. Cullen
  • Matthew Kowalik
  • Nicole M. Briglio
  • Harold J. Hoops
  • Zhouying Zhao
  • Michael A. Carpenter
چکیده

Aβ(1-40) coated 20 nm gold colloidal nanoparticles exhibit a reversible color change as pH is externally altered between pH 4 and 10. This reversible process may contain important information on the initial reversible step reported for the fibrillogenesis of Aβ (a hallmark of Alzheimer's disease). We examined this reversible color change by microscopic investigations. AFM images on graphite surfaces revealed the morphology of Aβ aggregates with gold colloids. TEM images clearly demonstrate the correspondence between spectroscopic features and conformational changes of the gold colloid.

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عنوان ژورنال:
  • International Journal of Molecular Sciences

دوره 10  شماره 

صفحات  -

تاریخ انتشار 2009